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LEIBOWITZ L-15 MEDIUM
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PHYSICAL-CHEMICAL CHARACTERISTICS
-Appearance: rosy and homogeneous powder
-Particles size: approximately 20 micras
-Solubility: clear solution at final concentration (1X)
-pH at 25ºC (without NaHCO3): 8.1 ± 0.30
-Osmolality: 320+5%
Storage: Store the powder or the final solution at 2 to 6°C. If these conditions are not maintained alterations may be observed, such as:
1. Colour change
2. Grainings
3. Insolubility
4. Alteration of pH
5. Incapacity to maintain its integrity under normal conditions.
Preparation: LEIBOVITZ L-15 MEDIUM is extremely hygroscopic and must not be exposure to the environment. Preparations with higher concentrations are not recommended due to the possibility of precipitate formation.
Measure out tridistillate water to approximately 90% of desired total volume of medium (15-20°C).
Add the powder and mix gently into equilibrium. DO NOT HEAT THE WATER.
Add antibiotics or other components if desired.
If necessary, adjust pH with HCl 1N or NaOH 0.1N, or also CO2.
Add tridistillate water to the final volume and filter the medium into sterile container using a 0.22m membrane filter.
PRESENTATION: Powder: bottles with powder for 1, 10 and 50 liters.
Liquid: Bottle with 100 and 500 ml.
STORAGE: 2 to 6°C
SHELF LIFE: 12 months
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Definition: It is a mixture of salts enriched with a large amount of amino acids. When compared to other media, it has more vitamins and other components that are essential for cellular growth. It was developed to be used in CO2 incubators, once its formulation does not have bicarbonate buffer.
Acts: It is a very nutritive solution for cell culture and, depending upon the use, does not need CO2 incubator to maintain pH.
Product specifications: It is applied for human and animal cell culture (lineage cells/HEp-2) and insect cells. It is available as powder and has the advantage to be stable for more than 24 months if well locked in freezer. Another advantage is the fidelity of results in programming cell culture at long term using the same lot.
Advantages: Because the buffer is based on salts, bases free of amino acids and galactose instead of glucose, it keeps a good self- control of physiological pH. Tested in at least three different cellular lineages, and its pH and osmolality are always determined. Each lot is only discharged if within proper limits, and the medium also promotes a great cellular growth.
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COMPONENTS OF LEIBOWITZ L-15 MEDIUM
INORGANICS SALTS |
mg/L |
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AMINO ACIDS |
mg/L |
CaCl22H2O |
185,00 |
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L-alanine |
225,00 |
KCL |
400,00 |
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L-arginine (free base) |
500,00 |
KH2PO4 |
60,00 |
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L-asparigine.H2O |
125,00 |
MgCl26H2O |
200,00 |
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L-cisteine (free base) |
120,00 |
MgSO4 |
97,67 |
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L-glutamine |
300,00 |
MgSO47H2O |
------ |
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Glycine |
200,00 |
NaCl |
8.000,00 |
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L-histidine (free base) |
250,00 |
Na2HPO4 |
190,12 |
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L-isoleucine |
125,00 |
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L-leucine |
125,00 |
VITAMINS |
mg/L |
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L-lysine |
75,00 |
D-Pantothenic Acid |
1,00 |
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L-methionine |
75,00 |
Choline chloride |
1,00 |
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L-phenylalanine |
125,00 |
Folic acid |
1,00 |
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L-serine |
100,00 |
Inositol |
2,00 |
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L-threonine |
300,00 |
Pyridoxal.HCL |
1,00 |
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L-tryptophan |
20,00 |
Nicotinamide |
1,00 |
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L-tyrosine |
100,00 |
Riboflavin |
0,10 |
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L-valine |
200,00 |
Thiamine |
1,00 |
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OTHER COMPONENTS |
mg/L |
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D(+)
galactose |
900,00 |
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Phebol red |
10,00 |
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Phebol red |
550,00 |
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